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Prep and dac scale up calculators phenomenex uhplc, hplc. Caffeine standard solution for hplc system qualification solution for gradient test. In order to translate a method from analytical to uhplc and to find out how much time and solvent will be saved we have produced a handy calculator to aid you. Standard samples for hplc system qualification 119158. Translations were made using a microsoft excel spreadsheet and the equations. Silicycle is a worldwide leader in the development, the manufacturing and the commercialization of high value silicabased and specialty products for chromatography, analytical and organic chemistry. Back pressure stays constant if column length stays constant. Sigmaaldrich hplc calculator appsupelco is a division of. Translating methods from hplc to uhplc chromatography online. There are quite a few free tools on the web to help you use hplc effectively. The ascentis family of columns is the fourth generation of hplc column technology from supelco scientists. Standard samples for hplc system qualification set 3. Ascentis columns are bonded on high purity, 100 angstrom silica including 3. Requires a number between 1 and 500 internal diameter mm.173 866 1359 572 794 842 882 1227 653 1413 1181 1360 1338 521 349 433 361 794 638 886 838 1249 1232 1174 576 582 848 371 889 1072 1237 882 744 806 269